Callus Induction And Screening Of Phytoconstituents In Gymnema Sylvestre ( R.Br)

warning: Parameter 1 to theme_field() expected to be a reference, value given in /home/blatter1/public_html/drupal/includes/ on line 170.


Diabetes is a chronic metabolic disorder with devastating long term consequence and affecting large percentage of world population. Gymnema sylvestre (R.Br) (GUDMAR) belonging to the family Asclepiadaceae causes hypoglycemic action and is traditionally used in the treatment of diabetes. It is an ingredient of popular herbal tea and ayurvedic preparations. It is marketed in the form of powder, tablets & capsules. Products of GYMNEMA, as it is of herbal origin does not produce any harmful effects where as synthetic medicines are known to produce some side-effects.

GYMNEMA is a rare plant and the fully grown plants have to be sacrificed for the preparation of these formulations. It involves lot of labour, expenditure and time. With an aim of overcoming these difficulties the present study was undertaken.

Plant Tissue Culture is a technique which can provide a solution to these problems.

Tissue culture of Gymnema sylvestre using various ex-plants was done. MS medium supplied with exogenous auxins and cytokinins was used. Within 15 days from the date of inoculation profuse callus formation was observed. The nature of the callus is heavy, granular and initially it was green later on it became dark brown in colour and still it is multiplying.

Along with many other chemical compounds the major constituent of Gymnema sylvestre is a glycoside which has suppressive activity of absorption of sugar. Detection of phytoconstitutents like Glycosides and alkaloids is done by Precipitation test, Chromatography and Gravimetry method. The tests have shown that leaves of mature plants and Calli produced by tissue culture contain nearly same amount of Glycosides and alkaloids. As it provides a method for obtaining pure material free from any contamination this method is definitely better than the plant source. In addition it is easier to extract phytoconstituents from callus tissue. Above all as plants are not destroyed it provides environmental protection.